FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_A01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_A01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3882333
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG14350.023.2396325
GTATTAG29850.021.6371381
CTAGGAC10950.020.5244183
CTATGCA23750.019.841699
GGACCGC4904.1472958E-1019.2340436
CCTAATA29700.019.040252
CTAATAC32800.019.0085033
GTATAGA25850.018.809121
TAGGACA19350.018.3585534
GTAGGAC9150.018.2234273
GCACGCC13600.018.1246199
TATACAG32800.018.1243865
TAGACCG22300.017.880565
TAGGACC15200.017.6474294
GTACTAG17850.017.4817311
CCTACAC11600.016.874433
TATACTG36700.016.7909855
GTCCTGG18650.016.7318441
ACTATGC33800.016.7302048
ACCGCGC5703.1122909E-916.5345278
Produced by FastQC (version 0.11.5)