FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4949417
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG48900.018.685
GTATTAG35800.016.217141
GTATAGA36500.014.7131681
ACTATGC40050.014.6621518
CTAATAC42350.014.379413
ATACAGG38350.014.1778736
TAATACT72100.013.9763814
GTATATA83700.013.8727271
CTATGCA34400.013.2774559
TATATAC88350.013.1289093
TATAATA100750.013.0962182
TACTATG40500.012.8882127
TTATACT56050.012.6755154
ATAATAC74050.012.6293163
GTATAAA62200.012.48420051
CATATAC51250.012.4481273
GTATATG48550.012.4066981
TATTATA100000.012.3969742
TATACCG23750.012.2098855
TAGGTAG19600.012.2059915
Produced by FastQC (version 0.11.5)