FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4336609
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG32400.024.1656465
TACAGGC23200.022.1865637
ATACAGG27100.021.6688256
GGTGCGA25600.021.522537
ACAGGCA29100.018.684788
GTCTAGT19550.018.1890411
AGGTGCG31800.018.0102466
TATAGGT35100.017.9695343
GTATTAG23900.017.9149111
ATATACA53650.017.837294
ATACTAT31650.017.1793696
ATTATAC27650.016.518483
TATATAC54700.016.4345953
CCTCGCG8600.016.017329
ATAAGGG29550.015.9470573
TAGGTGC38300.015.9001115
TTATACT23800.015.5352374
ATAGGTG42400.015.3886794
AATGCGC20500.015.2072019
CAGGCAA35900.015.1461259
Produced by FastQC (version 0.11.5)