FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_2554.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_2554.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5093734
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG21800.021.2838295
GTATTAG43250.021.14511
CTAATAC50650.019.6095373
TATACAG68350.018.3499075
CTATGCA43900.017.175279
ACTATGC55200.016.8111388
GTATATA127500.016.7933431
TAATACT96250.016.1943664
CCTAATA58250.016.1807732
TAGTCAG25600.016.1424589
GTATAGA46800.016.1291981
ACCTAAT56100.015.9135681
TATATAC139800.015.8686693
TACTATG58250.015.4330597
CTTACAC24850.015.1706163
ATACTAT87600.015.1451386
TATACCG28500.014.7539855
GTATAAT68250.014.4631051
TATACTG66050.014.378875
ATACAGG44950.013.8705936
Produced by FastQC (version 0.11.5)