FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_2553.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_2553.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3194053
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACAGG34900.021.188476
TATACAG48250.021.0356335
GTATTAG28000.020.474291
TAGGTAG13550.020.331495
TACAGGC21150.020.2239747
TATACCG19000.019.0783715
GTATATA87850.018.9161831
CTAATAC29850.018.7012773
ACAGGCA25950.017.6006248
GTATAGA30650.017.283521
CCCTATA21350.015.2805692
TATATAC96150.015.0047473
CTACCCC9750.014.87134554
TAGTCAG17550.014.4584799
TTAGGTA26450.014.2528784
GTATTAA35650.014.0452451
TCTATAC31200.013.9418863
GTGTAAG15650.013.9106271
TCTACAC23200.013.7495853
TACAGCG13750.013.7086767
Produced by FastQC (version 0.11.5)