FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_2552.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_2552.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2487286
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG12050.022.8624115
ATACAGG27900.022.0871776
CTAATAC25000.021.1693883
TATACAG38250.020.8490725
GTATTAG22250.020.2223971
TACAGGC18400.019.3065097
TTAGGTA18000.018.930014
GTATATA71600.018.4471531
TCTATAC25400.017.696343
CCTAATA29850.017.2444042
TATACCG14100.016.4534265
ACAGGCA22850.015.8638758
TACTATG31400.015.7001847
ACTATGC33750.015.6810288
GTGCGCG5102.623965E-715.6371189
TATATAC80100.015.5675913
GTATAAT30500.015.4662311
GTAGTCA16150.015.2627188
GTATTAA29650.015.1753241
TATATAG48850.015.1377425
Produced by FastQC (version 0.11.5)