FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_2551.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_2551.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10402538
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATAGGTGCGACGTGAACAGTGAGCTGTATGTGCGCAGCTCGCGTTGC241490.2321452707022075No Hit
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG223820.21515903138253378No Hit
GTATGTGCGCAGCTCGCGTTGCATTTTCGGAAGCGCTCGTTTTCGGAAAC213940.20566134918228607No Hit
GTATAGGAACTTCAGAGCGCTTTTGAAAACCAAAAGCGCTCTGAAGACGC149840.14404177134464685No Hit
TTACAGTCCGGTGCGTTTTTGGTTTTTTGAAAGTGCGTCTTCAGAGCGCT149150.14337847167681578No Hit
CTATATAACCTACCCATCCACCTTTCGCTCCTTGAACTTGCATCTAAACT139860.134447958757757No Hit
ATCCCATTCCATGCGGGGTATCGTATGCTTCCTTCAGCACTACCCTTTAG115610.11113633999702766No Hit
CTTCAAAGCGTTTCCGAAAACGAGCGCTTCCGAAAATGCAACGCGAGCTG112990.10861772386700246No Hit
GTACAAATAATGCGCGGCCGGTGCATTTTTCGAAAGAACGCGAGACAAAC105030.10096574509028469No Hit
TTCCTATACTTTCTAGAGAATAGGAACTTCGGAATAGGAACTTCAAAGCG104490.1004466410024169No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC98750.035.1662337
ATACAGG126150.032.010716
TATACAG133900.031.7281285
ACAGGCA111800.031.3853388
GGTGCGA137100.027.6561457
GTGCGAC138550.027.5234248
AGGTGCG142150.026.9286446
TATAGGT154700.025.447023
ATACTAT106550.020.752666
TAGGTGC195750.019.8884055
ATAGGTG194350.019.8451564
TATATAC227050.019.4456833
TAATGCG77450.019.3763588
TGCGACG196100.019.3356069
CAGGCAA188750.019.320349
GTGCGCA181700.019.0721845
GCGCAGC184250.018.8475727
TCAGCGC55800.018.709226
TGCGCAG185850.018.6853126
CCCTAGA69100.018.484821
Produced by FastQC (version 0.11.5)