FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_2550.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_2550.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27522404
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG125500.025.6484815
GTATTAG243050.021.9766981
TATACAG356150.019.7248295
CTAATAC287150.018.8091453
TAGTCAG161600.017.6312169
CTATGCA261550.017.3520479
ATACAGG250950.017.275736
TACTATG333150.016.9519637
GTATAGA265300.016.9329911
ACTATGC347750.016.573728
TACAGGC160950.016.5309377
ATACTAT448550.016.5181886
GTATATA659250.016.2926541
TTAGGTA220400.015.8546654
CCTAATA308450.015.8182992
TATTAGG290500.015.672672
ACCTAAT293450.015.4323021
TAATACT526750.015.2633074
TATATAC717000.014.9443453
GTAGTCA195200.014.8930838
Produced by FastQC (version 0.11.5)