FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_2549.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_2549.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11439112
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG129290.11302450749673576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG98650.017.647051
GTATATA254950.017.5260351
TATACAG149150.017.1100565
TATACTG130800.016.461935
GTATAGA111950.016.0040861
CTAATAC111500.015.0199733
TCTATAC107200.014.8785283
TATACGG76650.013.8093355
TATATAC268450.013.3412213
TAGGTAG59800.013.33593755
GTATAGG86900.012.9380831
CATATAC148600.012.8800393
CTATACA107950.012.6260424
ACTATGC103400.012.4804878
TATATAG187000.012.4450175
TAATACT194350.012.3847024
CCTAATA117500.012.3402562
TATACCG70250.012.2809715
GTATATG150100.012.22637751
CTTACAC62400.012.0831673
Produced by FastQC (version 0.11.5)