FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_2548.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_2548.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15294773
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG72500.021.7989085
GTATTAG131900.020.082551
TATACAG193300.017.327125
CTAATAC151700.017.1086833
GTATATA363600.017.0253471
GTATAGA143650.016.2675081
ACTATGC148800.015.3956358
TATATAC396550.015.0459973
TAGTCAG82900.015.0421069
TATACTG188000.014.8463315
CTATGCA128550.014.5506499
TAATACT269750.014.5396824
CCTAATA173300.014.4325292
TATACGG99450.014.2878715
GTATATG194650.014.1677221
TATACCG84500.014.15609555
GTATAAT218200.013.8359631
GGTAGTC101650.013.7646827
ATACTAT266600.013.3789246
TCTATAC140200.013.2893653
Produced by FastQC (version 0.11.5)