FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_2547.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_2547.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1224060
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG15600.023.2350065
TACACCG4154.5474735E-1122.708725
GTATAGA11600.020.0177561
TATACGG7100.019.3995935
GTATTAG9500.019.0958841
AGGTCGC2000.007921332518.1233046
TAGGTAG5301.1805241E-917.7813555
ACCGCGC2050.00913245817.6812748
ATACAGG11700.017.3488036
ACCGACC3002.4191535E-416.9150858
GTATATG14850.016.6140611
GTACACT7451.8189894E-1216.5420784
GTCCTAA6154.7293724E-1016.5187151
TACAGGC7051.0913936E-1116.4523627
GTATAAT16900.016.3162461
ACCTAAT12950.016.2499331
TCCTAAG5854.3855835E-916.1096042
GGACTGC5002.0948573E-715.9485086
TACGGTG6851.2914825E-1015.8744277
TTAGGAC5503.5839548E-815.8167033
Produced by FastQC (version 0.11.5)