FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_2546.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_2546.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18680259
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG87050.027.2332885
GTATTAG175350.021.5639131
CTAATAC204450.020.7439293
TACAGGC112600.020.538727
TATACAG246700.020.511975
ATACAGG178650.020.4526046
ATACTAT300300.018.878726
TACTATG233950.017.6943327
TTAGGTA143550.017.575124
CTATGCA192250.017.4599869
TAGTCAG127650.017.3224079
TAATACT372950.017.300624
CCTAATA213100.016.7381482
GTATATA424000.016.4666351
GTATAGA183600.016.4443341
TATATAC464300.016.3482113
ACCTAAT197700.016.3361231
TATACCG95800.015.9675415
GACTAGG78650.015.8544368
ACTATGC275450.015.7917258
Produced by FastQC (version 0.11.5)