FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_2545.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_2545.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64656365
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG516350.017.105211
TATACAG697450.016.8599935
TAGGTAG266200.016.1225345
GTATAGA551700.015.9565811
CTAATAC580600.015.7330573
GTATATA1255500.015.3876871
TATACGG355200.014.4504015
ACTATGC554150.014.4300158
TATACCG337250.013.6932685
TATATAC1341250.013.5886143
TCTATAC542050.013.4949543
GTATATG724450.012.8929451
CATATAC736550.012.8349733
ATACAGG518200.012.7310316
CTAGGAC179000.012.6363363
TATACTG732800.012.5445025
TAGACAG370550.012.3061855
TAATACT1046300.012.2779864
GTGTAAG307550.012.1763171
CTATGCA488100.012.0757329
Produced by FastQC (version 0.11.5)