FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_2544.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_2544.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17111600
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG136300.017.4644661
TATACAG173650.017.3259625
TAGGTAG73350.017.1978115
CTAATAC148050.016.1105943
GTATAGA146200.015.5372551
GTATATA305250.015.2635911
TCTATAC134000.014.3372263
CTATGCA125450.014.1588629
ACTATGC153050.014.068488
TATTAGG151750.013.7114182
CCTAATA154000.013.6993952
CTAGGAC49950.013.6432063
ACCGCGC27650.013.6342828
TATATAC328800.013.5822913
GTGTAAG79150.013.570261
TATACCG83900.013.5663115
TATACTG182500.013.4269685
GTATATG172100.013.1568241
TAATACT258250.013.0257214
CTTACAC76850.013.0184513
Produced by FastQC (version 0.11.5)