FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_2543.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_2543.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51583117
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG364150.016.8806041
CTAATAC403750.015.8192773
GTATAGA379600.015.5243961
TAGGTAG197200.015.1465015
ACTATGC370000.014.6953478
TATACCG223550.014.6908035
TATACAG426350.014.5215715
GTATATA744450.014.2136911
TCTATAC376150.013.6456883
CTATGCA306150.013.5691839
TATACTG532150.013.5553595
CCTAATA411400.013.4635472
CTATACT377100.013.3229364
TATATAC794600.013.1564873
TAATACT671300.012.927094
TAGACAG273800.012.8684095
TATACGG246450.012.8256585
ACCGCGC81450.012.8171588
ATACTAT603700.012.5852576
GTATATG472500.012.5335251
Produced by FastQC (version 0.11.5)