FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4188222
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG16650.021.3356325
GTATTAG31750.019.6594451
CCTAATA34500.017.6520122
ACTCCGC16200.016.5580948
CTAATAC34250.016.5104053
TATACGG19350.016.4852435
TATACAG39900.016.3528445
TAGTCAG17050.016.1580169
TATAATA80050.015.9398912
TAATACT57700.015.454434
ACCTAAT35350.015.1935341
CTATGCA29300.015.0935399
TACAGGC19700.015.0883347
ATTATAC45250.015.0602773
TACTATG40000.015.0432537
GTATATA68900.014.9584171
ATACTAT51450.014.5136126
TATTATA82400.014.4294812
TATATAC74950.014.4124663
ACTATGC39850.014.3721738
Produced by FastQC (version 0.11.5)