FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_1773.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_1773.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5277879
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACAGG48000.022.8058686
TATACAG66250.022.3231355
TACAGGC32350.020.1687567
TAGGTAG19150.019.6854555
GTATTAG42950.019.6014121
GTATATA119450.017.3161471
GAGCGCA12800.016.9919329
TATATAC131100.016.920983
ACAGGCA40350.016.7089948
CTAATAC48550.016.1265453
ATTATAC72600.015.1779743
ATATACA125750.015.1619314
TATACGG30550.014.9499675
TAATACT92550.014.4127944
GTATAAT67750.014.3544991
GTATAGA49100.014.337811
TATACTG60250.014.0779595
TTATACT59600.013.8664544
ATACTAT80900.013.8001286
CAGGCAA47900.013.470579
Produced by FastQC (version 0.11.5)