FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31879076
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG74850.018.0951965
GTATTAG139400.017.2207761
TATACAG199700.017.1189615
ACTATGC160450.016.3844038
GTATAGA170400.016.1734051
CTATGCA130350.016.0564889
GTATATA334900.015.6570741
TAATACT307750.014.591744
CTAGGAC47500.014.0283853
TACTATG167200.013.8604547
CCTAATA181450.013.7495632
TATTAGG155550.013.1948322
TAGACCG98150.013.0616025
TATACTG197350.013.0288095
TATACGG93700.012.9089345
TCTATAC161050.012.8623053
TATATAC387250.012.81187253
ATACAGG144050.012.6692536
CTAATAC249300.012.521853
TACACCG63200.012.4917385
Produced by FastQC (version 0.11.5)