FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_H12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_H12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2646986
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG2701.651988E-724.1651829
CTATACT11850.023.250594
GTATAGA14300.019.2907871
TACACCG6450.019.1098795
ACCGTGC5056.202754E-1018.6622188
CCTATAC9750.017.1037753
CCGCGCG3905.6222143E-616.7297389
GTATATG16200.016.5801731
TCCTAAG8950.016.2221832
CTAGTAC9400.016.1979543
CTAGGGG3153.5069245E-416.1122513
TATACAG15400.016.0076265
TAGCACG5002.0936386E-715.9511274
TCCTATA10950.015.9110732
GGATAGA14250.015.7924391
CTTACAC9200.015.7619853
GTACTAG9300.015.6116721
ACTGATC11850.015.2944188
GTATAAC14800.015.2055581
TATAGGG7202.6921043E-1015.1238072
Produced by FastQC (version 0.11.5)