FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_H11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_H11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3373896
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG28700.020.995551
TATACAG39950.019.7815885
TAGGTAG16100.019.3639535
GTATATA77500.018.360541
CTATACA29300.017.816254
TCTATAC31800.017.327583
GTCCTAA14300.016.2459471
CCGTGCG3655.968773E-515.8897259
GTATAGA31750.015.7774371
CTAGGAC9750.015.6158663
GTACTAG20000.015.6088021
TAGACCG15500.015.4359625
TATACTG44700.015.0843585
TATACCG19800.015.0130895
GTCCAGA18900.014.9807621
GTATAAG28250.014.9052971
TGTACCG12250.014.7963755
TATATAC81550.014.7582733
TAATACT54250.014.7009144
ACAGCGC9950.014.5722338
Produced by FastQC (version 0.11.5)