FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_H10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_H10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750958
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGAGCG1551.4645739E-632.7413337
GCGTAGC1450.001226290124.9995429
GAGGGCG1600.002174881322.6558349
ACGCGCA1600.002174881322.6558349
CAGAGCG2252.6507829E-522.5551419
TATAGGG2603.1752497E-622.3295842
CACACCG2303.13951E-522.0662824
CGTACAC2003.1010315E-421.7510493
ATCTAGG2104.2842975E-420.7346131
TAGAGAG2806.084403E-620.7152825
TACACCA4601.7644197E-1020.4901185
CATGCAC2155.052879E-420.2335343
ACTTAGC1800.00430654720.138528
ACTATGC4402.2610038E-919.7723648
AGTGTCC2205.902217E-419.7723648
GTATAAA6250.019.7393511
CACGACT1850.005044320619.5955414
GCTATAC2608.070904E-519.5201723
GTCCTAT2701.0706271E-418.8147431
CTAAGCA2701.0783157E-418.7972034
Produced by FastQC (version 0.11.5)