FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_H09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_H09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257757
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG10700.035.913965
GTATTAG19050.029.3430981
TAGTCAG11200.029.1284669
GACTAGG7350.027.6181038
GGTAGTC13250.027.3575537
CTATGCA17250.026.0570819
TATACCG12250.026.0428095
TATTAGG21050.025.8654122
CTAATAC22250.025.7435443
GTATAGA22700.025.2645441
ACTATGC21500.023.9410428
TAATACT32750.023.9102274
CCTAATA21650.023.4720132
TATACTG28900.022.5795825
TACTATG22900.022.4773987
GTGTAGG7150.022.3371051
AGACTAG9400.021.5950077
GTCCTAA11150.021.4856911
ACCTAAT21700.021.0761381
TAGACCG15550.020.9823115
Produced by FastQC (version 0.11.5)