FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_H08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_H08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3177921
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG25750.031.849781
TAGGTAG16800.031.0735515
TAGTCAG16050.028.0050589
GGTAGTC19850.025.2004577
TATTAGG30550.023.0444182
TATACAG34900.022.021565
TCTATAC28250.020.0190663
TTAGGTA27250.019.6894174
CCCGTGC3001.1071643E-519.3325238
GTCCTAG8300.019.2375551
CTAATAC26150.019.1313253
TAATACT46950.018.6860474
TATACTG40550.018.5955985
GTAGTCA25150.018.4485328
GTATAGA30450.018.353071
AGGTAGT29750.018.2765466
CAGCGTG9950.018.2153179
CTATACT30200.018.0061974
ACCGCGC5301.1823431E-917.7822748
CATACAC19900.017.488633
Produced by FastQC (version 0.11.5)