FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_H06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_H06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2842410
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA22600.022.4829751
GTATTAG23250.022.1666261
CTAATAC23900.021.841713
TAGGTAG10550.021.3040055
GGTAGTC12200.020.7987547
TAATACT36350.020.743454
TATTAGG20800.019.8918762
TAGACCG15400.019.7733655
CCTAATA23950.019.7002642
GTATAAG23250.019.3567711
TACTATG24400.019.3131287
TCGGGAC3051.2759405E-519.0170083
TACACCG10300.019.0054685
ACTATGC27350.018.8204568
TATACAG26900.017.788685
CTATGCA21450.017.5753979
GTATTAT39950.017.4428981
GTCTAGG14200.017.3802011
AGACCGT17700.017.2030376
CTAGACC17300.017.1826364
Produced by FastQC (version 0.11.5)