FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_H05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_H05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946683
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG14950.023.7932551
TAGGTAG7750.022.4523355
GTATTAG14200.021.9826011
TATACCG9200.021.277865
CTAATAC15400.020.7149543
TATACAG17550.020.2428175
CTATGCA10700.019.6491579
GGTAGTC8300.019.2165017
TACACCG6850.019.051715
GTGTAGG5801.0913936E-1118.7742351
CCGCGCC2359.205521E-418.5102849
GTCGTAG5157.912604E-1018.3246251
TATTAGG14500.018.0232662
TATACTG19350.017.9850755
GTGTATA13350.017.9445111
GTCCTAG4959.616997E-917.5984751
GTATAGA13550.017.14391
CTTACAC8550.016.9596123
TCTATAC14200.016.8491343
AGACCCG5201.7951606E-816.7313085
Produced by FastQC (version 0.11.5)