FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_H02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_H02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644739
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGGC2151.2732926E-1137.091149
CCTACAC1751.0066469E-733.1488843
GACTAGG2206.220944E-1032.952878
GTATTAG5300.031.4973071
TATACTG7750.029.940935
TAGGTAG3001.8189894E-1129.0052745
TAGTCAG2853.2196112E-1027.9810359
CTGCACG1358.081979E-426.8504899
CTAAGGC3451.07320375E-1025.221983
CTAGGAC1450.001224670725.0045493
AGACTAG3052.0329026E-823.7692837
TAAGTCG1851.8274298E-423.5177925
CCTAGAC3203.3744072E-822.660373
CCAGTCG1600.002174959822.6550989
GCATAGG2603.1704494E-622.3325391
ACCGTGT1952.6142452E-422.306568
GGTAGTC3604.84215E-922.1516517
ATCCTAC3304.6286004E-821.9941671
CACCGTG2353.708002E-521.5946467
GTATAGG4409.640644E-1121.4443131
Produced by FastQC (version 0.11.5)