FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_H01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_H01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7367971
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG26950.021.2546585
GTATTAG50950.020.8002831
CTATGCA41350.020.1625849
CTAATAC61600.020.0103193
CCTAATA51600.018.7095172
TAGACAG40500.018.2612695
TAGTCAG26700.017.9207679
ACTATGC57100.017.7752538
TATACAG54850.017.4495035
TATACTG66150.017.428225
TATACCG32650.017.3219685
TATTAGG50450.017.265562
GTATAGG43600.017.1479171
TAATACT85450.017.1405644
ATACTAT69750.017.0460416
TACACCG23500.016.9699525
ACAGTGC29400.016.7681628
GTATAGA52550.016.5755941
TACTATG55700.015.8791967
TAGACCG38050.015.2447785
Produced by FastQC (version 0.11.5)