FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_G12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_G12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537094
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTTG2005.456968E-1239.8742077
GCTAGAC1354.9628215E-737.5953453
CTTAGCC1001.389993E-436.2526553
GCCCCGA850.002741128434.1169668
CCCTACA1301.638888E-533.5045431
CCCTCGG1102.4349398E-432.953898
TCCTTGC2558.731149E-1131.2738868
TAGACTC1652.3823522E-630.7598275
TACGTCC1703.0065785E-629.8551254
CCTTGCG1703.0087613E-629.8523469
ATCCTAC1504.356625E-529.0372711
GCACCCT1000.006069815729.0021255
CTAGACT1255.1484944E-429.0021234
TAGACCC1255.1484944E-429.0021234
TCCCTGC1255.151302E-428.9994228
CCGCGCG1255.151302E-428.9994229
AGACCGT1804.69537E-628.1938826
CCCTTAC1050.007657176327.6545431
ACATACG1050.00770257527.6210673
CCTATAG1358.074363E-426.8538173
Produced by FastQC (version 0.11.5)