FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_G11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_G11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4006801
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG17200.021.0762275
GTATTAG31850.019.3684371
GTATAAG34750.016.707841
CTAATAC36800.016.1553863
TATACTG53000.016.1420045
TATACAG43250.015.5900745
GTATATA82000.014.8689581
TAATACT63050.014.83392054
GTGTAGG12300.014.7509511
TATACGG24200.014.6802025
GTATACG28450.014.5404451
CCTAATA40050.014.4968152
GTATAGA36100.014.2736911
ACTATGC32700.014.1891528
CTGTGCG7758.3309715E-1014.0318149
CTATGCA30000.014.0162219
TTAGGTA35300.013.76104454
TCTATAC35550.013.6642723
CTTACAC19750.013.5826953
ACAGCGT17250.013.4488488