FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_G09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_G09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3037344
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG13450.028.5718235
GTATTAG25000.023.2315461
CTATGCA21750.021.9990889
GGTAGTC14550.021.425237
CTAATAC31300.019.4589463
GTGTAAG15050.019.2953031
ACTATGC27250.019.1551698
GTCCTAG7500.018.391641
CCTAATA26850.018.3862322
ACCGCGC4755.869879E-918.3150318
TAGTCAG15100.018.2442769
GTATAGG20050.018.1043851
ACAGTGC14700.017.7543688
TATACAG30300.017.708165
TATTAGG26800.017.6078642
GTATATA56300.017.4081841
TAATACT44450.017.2909344
CAGCGTG11400.017.1703419
GTCCTAA13350.016.8580971
GTGTTAG20750.016.7938881
Produced by FastQC (version 0.11.5)