FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_G08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_G08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4470798
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA37950.021.2358131
ACCGTGC10000.021.0237068
CCGTGCG4051.8875653E-819.6901459
TAGGTAG20950.019.3819665
GTATTAG37050.018.616291
CAGCGTG15650.018.065989
CTAATAC37900.017.7925243
TATACTG54400.017.7274635
ACTATGC38800.017.563358
GACCGTC21350.017.3174347
TAGACCG23200.017.1897095
GTACTAG22850.017.1579441
CTATGCA31550.017.0037069
TAATACT60900.016.9069334
CCGTCGC18100.016.8221689
TCTATAC38950.016.75443
AGACCGT29350.016.5492366
TATACCG23950.016.3486585
CTATACT38500.016.1968864
TATACAG45950.015.9378495
Produced by FastQC (version 0.11.5)