FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_G07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_G07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5170917
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG40500.022.0446451
TAGGTAG23000.020.1746065
TATACGG29600.018.1256265
TATTAGG47950.016.5002652
ACTATGC45500.016.4113858
TAGTCAG23050.016.3550479
TTAGGTA40000.016.1318054
CTATGCA39150.016.110429
CTTACAC23900.016.077963
CTAATAC49900.015.9825153
ACCGTGC10600.015.7304588
GACCGTC24000.015.405597
GTCTAGG21700.015.38691
GGTAGTC28500.015.2625047
TATACCG28050.015.250085
GTATAGA46200.015.2399241
TATACAG60550.015.2069665
GTCCTAG14200.014.8239071
ATACCGT41150.014.798886
ACGGTGT30500.014.7370738
Produced by FastQC (version 0.11.5)