FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_G06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_G06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1577434
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG7400.026.452055
TATTAGG13250.024.0993212
GTATTAG13450.023.7409671
TAGTCAG6750.023.6290479
TAGACCG7850.023.0886025
GGTAGTC7400.021.5535227
TATACGG8600.021.075065
TCTATAC12850.020.8749713
GTATAGA13500.020.4276241
GCGTCCC1800.004308772320.1383919
CAGCGTG5555.456968E-1219.594119
GACCGTC7200.019.1314727
CCTACAC5005.4205884E-1018.8495333
GTATTAA15750.018.4309371
GTATAAG11100.018.3064041
TCCTAGG3201.9127123E-518.1429562
GTAGTCA11200.018.1245528
CTAATAC14650.017.815263
CTAGTAC8150.017.7909723
TTAGGTA12250.017.7546634
Produced by FastQC (version 0.11.5)