FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_G05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_G05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2128473
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG15600.024.6678431
TAGACCG10950.023.1756235
TAATACT27100.021.6717114
TAGTCAG8100.019.6908349
TATACAG17850.019.497575
GTATAGA17650.018.9231321
TCTATAC15400.018.832883
CTAGACT6700.018.3971964
CTAATAC17100.018.232653
TAGGTAG8750.018.2302285
GACCGCG3252.2053733E-517.8456787
GAGCGCG2450.001217754417.7546319
CCTAGAC8600.017.7050973
AGACCGT13500.017.1847296
CCCCTAT7200.017.1433751
GACCGTC8900.017.1062877
GTCCTAA8950.017.0363331
TATTAGG17600.016.9141852
ACTATGC16300.016.9013928
GTGTAGA16000.016.7904221
Produced by FastQC (version 0.11.5)