FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_G04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_G04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences952782
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG1851.6201375E-731.4025231
TAGACCG4000.029.0076875
GGGCCCG4350.028.3319197
CCGGGGC4750.027.4809654
GGGGCCC4500.027.387526
CCTAATA4950.026.4066642
CGGGGCC5350.025.7544885
CCTACAC2001.063212E-525.3817273
CTAATAC5750.025.2240753
GCACGCC2351.3309073E-624.6796179
TAGACAG3952.3646862E-1123.8670855
GTCTACG2451.8849914E-623.7121091
TAGGTAG3102.4107067E-823.3932955
AGCACGC3102.4188921E-823.385938
GCCCGGC4650.023.3859279
GTATTAG4750.022.9321041
AGACCGC2552.708035E-622.7439636
TTCCGGG6400.022.6932282
TCCGGGG5850.022.3136043
CTATGCA4256.184564E-1122.1753649
Produced by FastQC (version 0.11.5)