FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_G03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_G03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1480253
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC3352.1118467E-923.8053048
GACCGCG3702.6011548E-1023.5128567
CTAATAC7900.021.108433
AGACCGC5301.8189894E-1220.5182936
TACACCG3951.4126272E-820.1906745
CCGCGTA2205.9063145E-419.7721759
GGACCGC1900.00589014419.0784156
CCGCGCA3852.38766E-718.8306439
TATACAG7350.018.7422395
ACAGTGC5351.3387762E-917.6163318
GTATAGA8350.017.3882771
CCCTAAT4701.0194526E-716.9905681
ACTATAG5602.4538167E-916.8310243
GTACTAG6053.7107384E-1016.7990881
TCCCTAC3905.545251E-616.7529371
GGACCGT2600.00181262616.73036
GTCTACT6556.366463E-1116.6250511
ACAGTGT6658.185452E-1116.3529288
TAAGGGG4501.2229029E-616.111755
TAGGGGT3605.297642E-516.1117484
Produced by FastQC (version 0.11.5)