FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_G02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_G02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871827
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG3700.027.4402795
TATACCG4650.024.9533755
GTATTAG5850.021.0949761
ACTACGC2755.2120267E-621.0896848
CCTAATA8400.020.7404382
TACACAG4201.2623786E-920.720215
CTATGCA5950.020.7130819
GTCTAAG3901.2014425E-820.4745371
GTCCTAA4102.134584E-819.475781
GTATAGA7550.019.2295481
TACGGTG4553.434252E-919.1197667
CTAGGGT1900.005877574419.0844044
GTGTAGG3051.2608409E-519.0404031
CAGCGTG2307.963935E-418.9119439
ATACCGC4654.496542E-918.7085916
TATACGG5458.54925E-1118.629185
CAGTGCG2000.00791793918.1239459
CTAATAC8200.017.6879863
GACTAGG2500.001393743417.3989878
ACTATGC7950.017.3260368