FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_G01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_G01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3095378
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG13900.024.5155035
GTATTAG26050.020.3402961
CTATGCA21400.019.3103779
ACTATGC27400.017.9923558
CTAGGAC11850.017.7434223
GTATAGA25400.017.7174031
TATACAG33400.017.5831365
GTATATG33050.017.569551
CTAATAC29250.017.3512153
GGTAGTC16450.016.7473987
TAATACT47000.016.1975444
TATATAC57050.016.1400853
TAGTCAG15850.016.0091579
TACTATG31100.015.8517877
CCTAATA30050.015.700412
TCCTAAG16300.015.5855652
ATACTAT45050.015.4492046
TAGACAG21850.015.2638515
TTAGGTA25450.014.5291484
CCTACAC8501.4551915E-1114.5006593
Produced by FastQC (version 0.11.5)