FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_F12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_F12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612840
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCGC6750.032.2209858
CCCCCTA1306.4439303E-427.9146981
CTCCGCA7500.027.0656229
TAGGACC5150.022.5248014
TAGGTAG4659.094947E-1221.828475
GTATTAG10000.021.0476821
CAGTCCG2104.315027E-420.7147734
AACTCCG9800.020.7134887
TTAGACT9600.020.3911044
CCTACAC2857.100947E-620.3513573
ATAGGAC5800.020.000473
GTATAAT13800.019.4593751
ACAGTCC4102.1700544E-819.4504728
CCGCACG3001.10657875E-519.332599
ACTATGC9750.019.3325888
GTACCGC3852.3882603E-718.8304446
TACTATG10600.018.466277
CTATGCA8700.018.3326289
GTATATA16050.018.0880281
CCCTTAG5651.4006218E-1017.9839821
Produced by FastQC (version 0.11.5)