FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_F11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_F11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4897218
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG22450.020.9928195
GTATTAG40500.020.2552931
CTAATAC44250.017.6963733
GTATAGA45500.017.2316741
TAGTCAG22350.016.542969
TATTAGG46100.016.5349732
TATACAG52050.016.019575
GTATAGG32300.015.5082111
TAATACT75600.015.4410854
TATACTG62850.015.3433485
CCTAATA50450.015.10926152
GTATATA103050.015.0758181
CTATACT41100.014.99515154
CTATACA44550.014.9731754
TACACCG17000.014.92775
TCTATAC42950.014.8557053
CTAGGAC14200.014.8075573
GGTAGTC25500.014.7837197
TATACGG28000.014.7601445
TACAGCG19350.014.6118157
Produced by FastQC (version 0.11.5)