FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_F10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_F10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497356
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAG606.72392E-660.5322651
ATCGCGC800.002036644436.246338
GTCCTAA1402.7083131E-531.1308781
ACCGTGT1402.745821E-531.0682838
TCCTAAG1652.3479697E-630.8164232
ACCTAGA1453.4416924E-530.05741
CCTAGGA1255.0928496E-429.0554872
AGTCCCC1000.00607456428.9970658
GGGCGTG1504.397353E-528.9970637
CTAGATC2054.032281E-728.2926654
TTGTGCG1555.4987348E-528.0616769
CACTGCG1358.08175E-426.8491337
GTCTGGG1100.00957733526.4140762
CCTAGAT1100.00966703226.3636193
CTATCCT3108.56744E-1025.7257884
TACATAG2001.0640704E-525.3749835
GTCCACA2601.1213888E-725.1441691
GGACCCA1450.001226299824.9974696
GTATAGA3001.668559E-824.2129021
GTCCAGA2701.6152808E-724.2129021
Produced by FastQC (version 0.11.5)