FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_F09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_F09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4418361
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG17900.020.2542065
GTATAGA39900.017.8298721
GTATTAG35050.017.604591
GTATATA87550.017.0807151
TATACGG25900.016.7976955
TAGTCAG18150.016.7763399
TATTAGG41050.015.5619682
GACTAGG12750.015.3524648
TATACAG49000.014.7979715
CTATACT37100.014.4629224
ACCGTGC8702.1827873E-1114.1662278
GGTAGTC23550.014.1609187
TATATAC92300.014.062083
TACACCG15750.013.81143955
CTAATAC40200.013.7083683
GTATTAT74250.013.492041
CTAGGAC13650.013.280233
CCTAATA39950.013.0831022
TATACTG54000.013.0249545
TATAATA110650.012.9899892
Produced by FastQC (version 0.11.5)