FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_F08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_F08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4258269
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG33650.023.512511
GTATAGA37700.021.5642451
GACTAGG13100.017.7092638
GTATAAG34750.017.546161
TAGGTAG18250.017.4802385
TATTAGG38550.016.9463312
TAATACT60950.016.2968774
CAGGACC16050.016.2624134
CTAATAC38800.016.2571743
ACTATGC35450.016.1559568
TCCTAGG8550.016.1303982
GTCCTAG11350.015.9882781
GTATATA78500.015.9043681
TATACGG22100.015.7473135
TATACTG50300.015.7114425
GTATAGG26000.015.6340791
GTCTAGG17250.015.5693421
TAGGACA21100.015.4627794
ACCGTGT13200.015.3782158
TATACCG21700.015.3693545
Produced by FastQC (version 0.11.5)