FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_F07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_F07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8581103
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG37200.023.7794515
GTATTAG70350.021.8726981
CTATGCA66950.018.517079
CTAATAC88050.018.2813633
TATACGG52250.017.4851425
ACTATGC77200.017.4671638
GTATAGA80850.017.3263821
CCTAATA87200.016.8970222
GTATATA178200.016.3330481
TATACAG98150.016.3262675
TCTATAC80150.016.2837313
TAGACCG47050.015.873135
GGTAGTC48250.015.77679357
CTTACAC39450.015.6227263
TAATACT136700.015.5942234
TAGTCAG42400.015.5597379
TACTATG85700.014.7195717
CTAGGAC25800.014.6139823
CATATAC104700.014.3353613
ACCGTCG42100.014.2929798
Produced by FastQC (version 0.11.5)