FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_F06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_F06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40178
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTAG100.0070138853145.357061
CCGCTTG100.0070664394144.995019
ACAGGGA100.0070664394144.995018
TCTATAC100.0070664394144.995013
ACTGTGG300.001922452672.4975056
CCTGGGA350.003507223462.295882
TATATCG350.003542109462.1407245
TATAGAG400.00595054754.5088962
CTGGGAA400.006009653754.373133
TATAGTA450.0094796748.452352
TTCTGAT450.00957370148.3316737
TATATAG800.001989239636.3392642
ATCATAT800.001989239636.3392641
GGGGGGG45650.09.211075145
AAAAGGG3050.0058723667.130903145
AAGGGGG2450.0038773946.50998140-144
GCTTGAA4150.00106720845.2407837140-144
Produced by FastQC (version 0.11.5)