FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_F05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_F05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235513
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG10100.024.4072535
GTATTAG17050.022.5610851
TATTAGG18350.021.7538012
CTAATAC18750.020.8811243
CAGCGTG7600.020.0317349
GACTAGG6550.019.9225088
GTATAGA19700.019.8946381
TGTACCG7700.019.773795
GTCCTAA9200.019.722441
TATACAG19400.019.4340365
GTACCGC6101.8189894E-1219.0152916
ACTATGC17400.018.7489138
TAATACT29950.018.1562374
GTATATA35800.018.043281
GTATTAA21050.017.929151
TACAGCG10800.017.4526927
TACCGCG7550.017.2837667
ACTAGGC8400.017.2609049
GGTAGTC11000.017.135377
GTATAAG15750.017.0502071
Produced by FastQC (version 0.11.5)