FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_F02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_F02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1744903
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG7500.026.1019295
GTATTAG13400.022.213761
CTAGGAC4951.8189894E-1221.971323
TATTAGG15600.020.0118332
TAGTCAG8750.019.8849059
GGTAGTC9150.019.8079367
TATACCG9250.019.5960435
GTATATA33000.019.5802881
GACTAGG4603.939931E-918.9122758
TAGGACC6201.8189894E-1218.711064
TATACTG21250.018.083695
TTAGGTA13800.017.8636384
CTTACAC9150.017.4329813
TATACAG19200.017.3710775
CTATACT14700.017.2631824
GCTATAC7800.016.7320063
CTAGACT4901.6628655E-716.2767124
TATACGG9450.016.11235
GTATAGA16300.016.0345921
CCCTGCG4109.036521E-615.9139869
Produced by FastQC (version 0.11.5)