FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_E12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_E12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035412
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG6500.025.6901591
ATAGTGT7150.024.3348066
TAGACCG3301.7716957E-924.1681485
TGCTCCG1500.001493775824.1681485
TATAGTG7250.024.001475
GCACCGC1550.001808903823.386276
CCTGCAC3803.6379788E-1022.896143
TGCACCG2603.202642E-622.309065
CTATGCA4701.0913936E-1121.5949829
CAGCGCG2053.667436E-421.2187639
CTAAGGT3651.3593854E-719.8642314
GTATAAT9200.019.7289731
TAATACT10800.019.4687864
GTCCTAG2307.886825E-418.9398141
ACTATGC6151.8189894E-1218.8611228
CCCTAAT3852.350007E-718.8578241
TACTATG6650.018.533187
TAGTAGT8500.017.9128634
AGCCTAG2450.001205473917.7802351
GCTAGGA2050.00905256417.7079562
Produced by FastQC (version 0.11.5)