FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_E11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_E11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3600883
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGA7150.021.3198682
GTCTAAG17200.018.9913271
GTATTAG31100.018.4390281
CTAGGAC10800.018.12613
CTAATAC33650.017.4527683
GTATATA76100.016.8833981
CCCTAGG6154.7111826E-1016.5243431
TATACGG22500.016.4343325
TAGGTAG14700.016.2764995
ACTATGC30100.016.1372858
CTATGCA26550.015.8374639
TATACCG19000.015.6456865
TAATACT59100.015.3351094
CCTAATA36250.015.0184312
TATACTG45800.014.8808165
ATACTAT56700.014.8319116
TAGGACA18200.014.7399064
GTATAGA31550.014.7248851
TATACAG38750.014.5944355
GTATATG41100.014.4824921
Produced by FastQC (version 0.11.5)