FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_E10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_E10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105328
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCCC100.007032642145.338881
CCGCTTA259.364858E-486.99639
GAGTTAC451.17525466E-464.595061
CTTAGCA350.003524877662.2880972
CTATATG350.00355155762.169734
GCTATCC501.99768E-458.025083
TATTAGG400.00598039654.5020832
ACCCTTG400.00602559854.3985145
TCTCTAG400.00603693854.372698
CTTGGCG400.00603693854.372699
CTAAAAG553.1989286E-452.7500763
ATACTAA803.66091E-545.4184041
CCTTTCT657.308137E-444.6134839
CTATCCC700.001050097441.4464874
TACTCCT750.001474221538.6833885
TTCTGGG800.002005234136.334722
CTAATAT1001.3794962E-436.2656753
TCCTGTG800.002028789636.248467
CTATTCT850.002725122334.13244
TATTCTC1102.4153045E-432.9687965